DNA

Part:BBa_K5310021:Design

Designed by: Elias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-26)


T2 hairpin arm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure may not be as stable in vivo as it is shown to be in NUPACK and in vitro experiments.


Source

Designed by our team based on complementarity with miR-146a-5p and the rest of the Y hairpin as well as stability standards (thermodynamics, closing base pairs, loop size).

References